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Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
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A Ligand-Modulated Padlock Oligonucleotide for Supercoiled Plasmids.
Thibaut Roulon1, Claude Hélène1, Christophe Escudé1
1Laboratoire de Biophysique, INSERM U201 CNRS UMR8646 43, rue Cuvier, 75231 Paris Cedex 05 (France) Fax: (+33) 1-40-79-37-05.
Angewandte Chemie (International Ed. in English)
|May 2, 2018
Summary
Ligand-induced triple-helix formation enables efficient catenation of circular oligonucleotides to supercoiled plasmids. This supramolecular structure
Area of Science:
- Biochemistry
- Molecular Biology
- Supramolecular Chemistry
Background:
- Plasmid functionalization is crucial for various molecular biology applications.
- Achieving high-yield, specific DNA ligation remains a challenge.
Purpose of the Study:
- To develop a novel method for high-yield oligonucleotide-plasmid catenation.
- To explore the use of ligand-induced triple-helix formation for DNA manipulation.
Main Methods:
- Utilizing ligand-induced triple-helix formation to link circular oligonucleotides and supercoiled plasmids.
- Employing a triplex-stabilizing agent (TSA) to modulate noncovalent interactions.
Main Results:
- Demonstrated high yields in the catenation of circular oligonucleotides to supercoiled plasmids.
- Showcased the circular oligonucleotide as a noncovalent anchor for plasmid functionalization.
Conclusions:
- Ligand-induced triple-helix formation provides an efficient strategy for oligonucleotide-plasmid conjugation.
- This method offers a versatile approach for plasmid functionalization and supramolecular assembly.
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