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Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
Published on: August 6, 2012
Design of a Molecular Beacon DNA Probe with Two Fluorophores
Peng Zhang1, Terry Beck2, Weihong Tan1
1Department of Chemistry and UF Brain Institute University of Florida Gainesville, FL 32601 (USA) Fax: (+1) 352-846-2410.
Abstract:
Fluorescence resonance energy transfer between two fluorophores (F1 and F2 ) attached to the two ends of a molecular beacon DNA probe containing a hairpin structure can be used for quantitative DNA/RNA studies. Concentrations of target-DNA as low as 1.7×10-10 M could be determined with a commercial spectrometer by using coumarin and 6-carboxyfluorescein as the fluorophores. Measurements on the Förster energy transfer distance for the donor/acceptor pair can also be carried out using these DNA probes.
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