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Updated: Feb 11, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
Array-In-Well Epitope Mapping of Phage-Displayed Antibodies
Urpo Lamminmäki1, Gaurav Batra2, Petri Saviranta3
1Department of Biochemistry/Biotechnology, University of Turku, Turku, Finland. urplammi@utu.fi.
Abstract:
Novel affinity reagents, such as single chain (scFv) antibody fragments, can be generated by isolating them from recombinant protein libraries using phage display selection. A successful selection process against a target protein can produce a number of binder candidates among which the desired binders are identified by screening and characterization of individual clones. Obtaining information on the binding properties, such as the binding epitope, already during the screening step helps to choose the most useful candidates for further development at early phase saving time and resources. To this end, we describe here an Array-in-Well-based screening procedure to perform activity testing and epitope mapping for filamentous phage-displayed scFvs in an integrated manner with a single assay.
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