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Repressed memories are a psychological phenomenon where memories of traumatic events are unconsciously blocked from a person's awareness. This process occurs as a defense mechanism, protecting the mind from the emotional impact of distressing or painful experiences. For example, a person who has experienced childhood trauma may grow up with no conscious recollection of the event. In such cases, the memories are thought to be buried deep within the subconscious, inaccessible to the conscious...
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Related Experiment Video

Updated: Feb 11, 2026

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Toward tunable dynamic repression using CRISPRi.

Sungyeon Jang1, Sungho Jang1, Gyoo Yeol Jung1,2

  • 1Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk, 37673, Korea.

Biotechnology Journal
|May 2, 2018
PubMed
Summary
This summary is machine-generated.

Quantitative gene expression control using CRISPR interference (CRISPRi) was achieved by modulating single guide RNA (sgRNA) levels. This simplifies dynamic tuning of gene activity, overcoming previous limitations in CRISPRi applications.

Keywords:
CRISPRidCas9dynamic regulationsgRNAtunable gene expression

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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Biotechnology

Background:

  • CRISPR interference (CRISPRi) is a powerful tool for gene expression repression.
  • Its popularity stems from simple design rules and multiplexing capabilities.
  • Quantitative, dynamic control of gene expression using CRISPRi has been challenging.

Purpose of the Study:

  • To develop a method for quantitative and dynamic control of gene expression using CRISPRi.
  • To identify key factors influencing CRISPRi efficacy for precise gene regulation.

Main Methods:

  • Modulating the expression levels of CRISPRi components, deactivated Cas9 (dCas9) and single guide RNAs (sgRNAs).
  • Utilizing various constitutive and inducible promoters to control component expression.
  • Analyzing the impact of sgRNA expression levels on gene repression.

Main Results:

  • The expression level of sgRNA was identified as the critical factor for controlling CRISPRi.
  • Modulating sgRNA expression enabled quantitative tuning of CRISPRi-mediated gene expression.
  • This method allows for precise, dynamic control over target gene expression levels.

Conclusions:

  • sgRNA expression level is the key determinant for quantitative CRISPRi.
  • This approach offers a simple and effective strategy for dynamic gene expression modulation.
  • The findings facilitate broader applications of CRISPRi in various biological studies.