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Published on: April 12, 2024
Novel AKT1 mutations associated with cell-cycle abnormalities in gastric carcinoma
Souvik Ghatak1, Sylvine Lalnunhlimi1, Freda Lalrohlui1
1Department of Biotechnology, Mizoram University, Aizawl - 796004, Mizoram, India.
Aim:
The aim of this study is to identify the AKT1 gene mutation driven pathogenicity in gastric cancer for Mizo population.
Methods:
50 diffuse-type gastric tumors were analyzed for AKT1 exon 2 and 14 mutations. Cell-cycle aberration was analyzed in the AKT1-mutated samples and the stability of the protein as well as exonic splicing enhancer motifs were examined.
Results:
The novel mutations, 15553T >A and 25376C >G might affect the exonic splicing enhancers and silencers. Significant decline was observed in the S-phase population in the tumor cells with 15553T >A and 15579G >C mutations suggesting the arrest of G1 phase.
Conclusion:
The present study is a novel finding of the possible role of AKT1 mutations which might help to identify gastric cancer patients.
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