Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.9K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.9K
CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.4K
Overview
80.4K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.2K
Genomics02:02

Genomics

40.9K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.9K
Uncertainty in Measurement: Accuracy and Precision03:37

Uncertainty in Measurement: Accuracy and Precision

104.1K
Scientists typically make repeated measurements of a quantity to ensure the quality of their findings and to evaluate both the precision and the accuracy of their results. Measurements are said to be precise if they yield very similar results when repeated in the same manner. A measurement is considered accurate if it yields a result that is very close to the true or the accepted value. Precise values agree with each other; accurate values agree with a true value. 
104.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Genetic Engineering to Preclinical Safety: A Study on Recombinant Human Interferons.

International journal of molecular sciences·2025
Same author

Multilayer Nanocarrier for the Codelivery of Interferons: A Promising Strategy for Biocompatible and Long-Acting Antiviral Treatment.

Pharmaceutics·2024
Same author

Peptide hemolytic activity analysis using visual data mining of similarity-based complex networks.

NPJ systems biology and applications·2024
Same author

Cysteine-Based Perfluorinated Derivatives: A Theoretical and Experimental Study.

ChemPlusChem·2023
Same author

Fast, cheap and sensitive: Homogenizer-based RNA extraction free method for SARS-CoV-2 detection by RT-qPCR.

Frontiers in cellular and infection microbiology·2023
Same author

Hydrogel for the Controlled Delivery of Bioactive Components from Extracts of <i>Eupatorium glutinosum</i> Lam. Leaves.

Molecules (Basel, Switzerland)·2023

Related Experiment Video

Updated: Feb 11, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

7.0K

CRISPR-Cas9: A Precise Approach to Genome Engineering.

Jorge E Simón1, Ángel S Rodríguez1, Nelson Santiago Vispo1

  • 11 School of Biological Sciences and Engineering, Yachay Tech University, Urcuquí, Ecuador.

Therapeutic Innovation & Regulatory Science
|May 2, 2018
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas9 gene editing offers a cheaper, faster alternative to older genomic manipulation methods. This revolutionary technology is advancing disease genetic engineering and therapeutic applications, though regulatory principles are still developing.

Keywords:
CRISPR-Cas9NGSbacterial adaptive defense systemclinical trialsgenetic engineering

More Related Videos

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

4.0K
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
08:31

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Published on: June 20, 2019

14.8K

Related Experiment Videos

Last Updated: Feb 11, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
08:20

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Published on: November 3, 2020

7.0K
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

4.0K
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
08:31

CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

Published on: June 20, 2019

14.8K

Area of Science:

  • Genetics and Genomics
  • Biotechnology
  • Molecular Biology

Background:

  • Recombinant DNA technologies have advanced genomic manipulation but are often costly and labor-intensive.
  • Next-generation sequencing (NGS) technologies offer more accessible genomic study methods.
  • CRISPR-Cas9, an NGS-derived technique, provides a revolutionary approach to genomic modification.

Purpose of the Study:

  • To explore the origins, engineered variants, and behaviors of CRISPR-Cas9.
  • To present the diverse applications of CRISPR-Cas9 in biotechnology and human therapeutics.
  • To discuss the regulatory considerations surrounding genome correction technologies.

Main Methods:

  • Review of CRISPR-Cas9 origins from bacterial defense mechanisms.
  • Analysis of engineered variants and their functional behaviors.
  • Investigation of current and potential applications in biotechnology and medicine.

Main Results:

  • CRISPR-Cas9 enables efficient modification, regulation, and marking of specific genomic sequences.
  • Adaptations of CRISPR-Cas9 facilitate permanent editing of dysfunctional genes and correction of missing proteins.
  • Clinical trials and in vivo studies are actively exploring CRISPR-Cas9 applications.

Conclusions:

  • CRISPR-Cas9 represents a significant advancement in genetic engineering, opening new avenues for disease treatment.
  • The technology's potential in biotechnology and human therapeutics is vast and actively being investigated.
  • Regulatory frameworks are essential to address the security concerns associated with genome correction.