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microRNA-107 inhibits gastric cancer cell proliferation and metastasis by targeting PI3K/AKT pathway

Feng Cheng1, Zhijun Yang2, Fei Huang1

  • 1Clinical Laboratory, Ezhou Central Hospital, Ezhou, Hubei, 436000, China.

Microbial Pathogenesis
|May 2, 2018
PubMed

Insights

MicroRNA-107 (miR-107) acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation and metastasis. This study shows miR-107 targets BDNF, offering a potential therapeutic strategy for gastric cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Gastric cancer is a leading cause of cancer-related deaths worldwide.
  • Dysregulation of microRNAs (miRNAs) is implicated in gastric cancer development and progression.
  • miR-107 has been identified as a potential tumor suppressor, but its precise role in gastric cancer requires further investigation.

Purpose of the Study:

  • To investigate the effect of miR-107 on gastric cancer cell proliferation and metastasis.
  • To elucidate the molecular mechanism underlying miR-107's function in gastric cancer.
  • To evaluate the therapeutic potential of miR-107 in gastric cancer treatment.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess miR-107 levels.
  • Cell Counting Kit-8 (CCK-8) assay for cell proliferation.
  • In vivo tumor growth assays in mice.
  • Wound-healing and Transwell assays for cell migration and invasion.
  • Luciferase reporter assays to validate target genes.
  • Western blot analysis for protein expression.
  • Transfection with miR-107 mimics, negative controls, and si-BDNF.

Main Results:

  • miR-107 expression was significantly downregulated in gastric cancer cell lines (SGC-7901, MKN1) compared to normal cells (GES-1).
  • miR-107 transfection inhibited proliferation, tumor growth, migration, and invasion of SGC-7901 cells.
  • miR-107 directly targeted BDNF (Brain-Derived Neurotrophic Factor), reducing its mRNA and protein expression.
  • miR-107 overexpression inhibited the PI3K/AKT signaling pathway.
  • Restoring BDNF expression or inhibiting BDNF expression reversed the effects of miR-107 on proliferation and signaling pathways.

Conclusions:

  • miR-107 functions as a tumor suppressor in gastric cancer by inhibiting cell proliferation and metastasis.
  • miR-107 exerts its tumor-suppressive effects by targeting BDNF and subsequently inhibiting the PI3K/AKT pathway.
  • miR-107 holds promise as a potential therapeutic agent for gastric cancer.
Keywords:
Luciferase activityMetastasisTransfectionTranswell invasionWound-healing

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