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An antiserum to the C-terminus of the Alzheimer amyloid precursor recognizes a soluble 70 kDa protein
J Simpson1, C M Bladon, C M Yates
1Department of Pharmacology, University of Edinburgh, Scotland.
FEBS Letters
|September 12, 1988
Abstract:
A rabbit antiserum to the C-terminus of the putative brain amyloid precursor was used to probe Western blots of tissue proteins separated by SDS-PAGE. The antiserum specifically labelled a protein of approx. 70 kDa in the Tris buffer-soluble fraction of brain samples from rat, Alzheimer subjects, cases of young and old Down's syndrome, and age-matched controls. The 70 kDa protein was present in low concentrations in human liver and kidney, and was undetectable in human skeletal muscle. The 70 kDa protein may be a metabolite of the amyloid precursor.