Related Experiment Video
Updated: Sep 3, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
Characterization and cloning of gene 5 of Bacillus subtilis phage phi 29
Abstract:
Sequencing of the phi 29 DNA region [open reading frames (ORFs) 12, 11 and 10] between genes 6 and 4 of the mutant ts5(219) showed that a G in the wild-type phage had been changed to an A in the mutant at position 218 of ORF 10 indicating that this ORF corresponds to gene 5. ORF 10 was cloned in plasmid pPLc28 under the control of the PL promoter of phage lambda and, after heat induction of the Escherichia coli cells carrying the recombinant plasmid pGM26, a 12-kDa protein was overproduced, accounting for about 5% of the de novo synthesized protein. Introduction of a nonsense mutation in ORF 10 indicated that the latter codes for the 12-kDa protein. The predicted secondary structure, the hydrophilicity values and the antigenic regions of protein p5 are discussed.
Related Concept Videos
Lytic Cycle of Bacteriophages
Lysogenic Cycle of Bacteriophages
Bacterial Transformation
Viral Replication: Lytic Cycle
DNA Bacteriophages

