Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.6K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.6K
Methods of Documentation IV: Focus Charting01:26

Methods of Documentation IV: Focus Charting

1.8K
Focus Charting, also known as the focus charting system or "focus documentation," is a systematic documentation approach used in healthcare to organize patient information in medical records.
It typically involves three columns for recording information:
1.8K
Sensory Modalities01:15

Sensory Modalities

4.0K
Sensation typically is the process by which the sensory receptors and sense organs detect stimuli from the internal and external environment and transmit this information to the central nervous system for processing.
General senses refer to the broad category of sensory information detected by receptors in the body and can be further grouped into somatic and visceral senses. Somatic sensations include touch, pressure, temperature, and pain and are essential for navigating our environment and...
4.0K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

10.9K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
10.9K
Leaky Scanning02:28

Leaky Scanning

5.7K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.7K
Trigonometric Fourier series01:17

Trigonometric Fourier series

820
Fourier series is a foundational mathematical technique that decomposes periodic functions into an infinite series of sinusoidal harmonics. This method enables the representation of complex periodic signals as sums of simple sine and cosine functions, facilitating their analysis and interpretation in various fields, including signal processing, acoustics, and electrical engineering.
The trigonometric Fourier series specifically expresses a periodic function with a defined period T using sine...
820

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scalp salvage with acellular dermal matrix assistance for recurrent exposure of deep brain stimulation hardware: a case report.

Archives of craniofacial surgery·2026
Same author

Unclonable Three-Level Holograms for Two-Factor Visual Authentication via One-Step Cascade-Nanostructure Imprinting.

Nano letters·2026
Same author

HIV-1 protease cleavage sites detection with a quantum convolutional neural network algorithm.

Scientific reports·2026
Same author

Comparative connectomics reveals stage-specific gap junction rewiring that reshapes avoidance behavior.

bioRxiv : the preprint server for biology·2026
Same author

Confidence Set for the Cluster of a Spatial Scan Statistic.

Statistics in medicine·2026
Same author

Association Between Illness Severity Scores and Quantitatively Measured Brain Injury in Cardiac Arrest Survivors.

Journal of clinical medicine·2026

Related Experiment Video

Updated: Feb 11, 2026

Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes
09:20

Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes

Published on: February 8, 2018

11.4K

Through-focus scanning optical microscopy with the Fourier modal method.

Shin-Woong Park, Gyunam Park, Youngbaek Kim

    Optics Express
    |May 3, 2018
    PubMed
    Summary

    We developed a Fourier modal method (FMM) based through-focus scanning optical microscopy (TSOM) for reliable nano-scale metrology. This technique offers sub-nanometer measurement tolerance, validating the TSOM concept.

    More Related Videos

    Three-dimensional Optical-resolution Photoacoustic Microscopy
    08:31

    Three-dimensional Optical-resolution Photoacoustic Microscopy

    Published on: May 3, 2011

    18.9K
    Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound
    07:34

    Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound

    Published on: July 18, 2020

    3.5K

    Related Experiment Videos

    Last Updated: Feb 11, 2026

    Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes
    09:20

    Novel Photoacoustic Microscopy and Optical Coherence Tomography Dual-modality Chorioretinal Imaging in Living Rabbit Eyes

    Published on: February 8, 2018

    11.4K
    Three-dimensional Optical-resolution Photoacoustic Microscopy
    08:31

    Three-dimensional Optical-resolution Photoacoustic Microscopy

    Published on: May 3, 2011

    18.9K
    Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound
    07:34

    Delivery of Antibodies into the Brain Using Focused Scanning Ultrasound

    Published on: July 18, 2020

    3.5K

    Area of Science:

    • Optical Metrology
    • Nanoscale Measurement
    • Computational Optics

    Background:

    • Through-focus scanning optical microscopy (TSOM) is a novel non-destructive metrology technique.
    • The reliability and feasibility of TSOM are currently under investigation and subject to debate.

    Purpose of the Study:

    • To experimentally demonstrate and verify the nano-scale metrology capabilities of FMM-based TSOM.
    • To provide a numerical tool for achieving true nanometer-scale TSOM.
    • To elucidate the key elements for high-level consistency in FMM-based TSOM.

    Main Methods:

    • Implementation of Fourier modal method (FMM) for TSOM image acquisition modeling.
    • Experimental validation of the FMM-based TSOM system.
    • Inclusion of illumination parameters (incidence angle, polarization, coherence, NA) in FMM modeling.

    Main Results:

    • Stable nano-scale metrology was experimentally demonstrated, confirming TSOM's viability.
    • Precise agreement was achieved between calculated and experimentally measured TSOM images.
    • The FMM-based approach provides a robust numerical tool for nanometer-scale TSOM.

    Conclusions:

    • FMM-based TSOM offers sub-nanometer measurement tolerance, establishing its reliability.
    • Accurate modeling of optical parameters is crucial for high-fidelity TSOM.
    • This work provides a validated framework for advanced optical metrology.