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Updated: Feb 11, 2026

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
Published on: May 20, 2013
Note: Sensitive fluorescence detection through minimizing the scattering light by anti-reflective nanostructured
Supeng Xu1, Yanning Yin1, Ruoxi Gu1
1Key Laboratory of Precise Spectroscopy, School of Physics and Materials Science, East China Normal University, Shanghai 200062, People's Republic of China.
Abstract:
We demonstrate a new approach with fabrication of anti-reflective coating to substantially reduce the scattering light in an ultra-high vacuum during laser induced fluorescence (LIF) detection. To do so, the surface of the vacuum chamber in the detection region was blackened and coated with the special solar heat absorbing nanomaterials. We demonstrate that more than 97.5% of the stray light in the chamber spanning from near infrared to ultraviolet can be absorbed which effectively improves the signal to noise (S/N) ratio. With this technique, the LIF signal from the cold magnesium monofluoride molecules has been observed with an S/N ratio of ∼4 times better than without that.
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