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    Area of Science:

    • Reproductive Biology
    • Developmental Biology
    • Cryobiology

    Background:

    • Embryo cryopreservation is crucial for preserving genetic diversity and facilitating research.
    • Established protocols are essential for reliable long-term storage and distribution of valuable biological samples.

    Purpose of the Study:

    • To describe a widely adopted and highly effective embryo cryopreservation protocol.
    • To highlight the protocol's simplicity, effectiveness, and high success rates for various developmental stages.

    Main Methods:

    • Utilizes a controlled-rate freezer for cryopreservation of embryos.
    • Applicable to embryos at the two-cell to blastocyst stages.
    • Thawing process requires no special media or equipment due to specialized insemination straws.

    Main Results:

    • Successfully cryopreserved over 2 million embryos from thousands of strains.
    • Achieves embryo survival rates consistently greater than 90%.
    • Demonstrates ease of use and rapid staff proficiency with prior experience.

    Conclusions:

    • This protocol is a robust, reliable, and effective method for embryo cryopreservation.
    • Its simplicity and the integrated thawing components in insemination straws facilitate efficient distribution.
    • The high survival rates validate its widespread use in global repositories.