Related Experiment Video
Updated: Feb 11, 2026

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates
Published on: November 18, 2013
Facile Tumor Spheroids Formation in Large Quantity with Controllable Size and High Uniformity
Wentao Shi1, Jean Kwon1, Yongyang Huang2
1Department of Bioengineering, Lehigh University, Bethlehem, Pennsylvania, 18015, USA.
None:
A facile method for generation of tumor spheroids in large quantity with controllable size and high uniformity is presented. HCT-116 cells are used as a model cell line. Individual tumor cells are sparsely seeded onto petri-dishes. After a few days of growth, separated cellular islets are formed and then detached by dispase while maintaining their sheet shape. These detached cell sheets are transferred to dispase-doped media under orbital shaking conditions. Assisted by the shear flow under shaking and inhibition of cell-to-extracellular matrix junctions by dispase, the cell sheets curl up and eventually tumor spheroids are formed. The average size of the spheroids can be controlled by tuning the cell sheet culturing period and spheroid shaking period. The uniformity can be controlled by a set of sieves which were home-made using stainless steel meshes. Since this method is based on simple petri-dish cell culturing and shaking, it is rather facile for forming tumor spheroids with no theoretical quantity limit. This method has been used to form HeLa, A431 and U87 MG tumor spheroids and application of the formed tumor spheroids in drug screening is also demonstrated. The viability, 3D structure, and necrosis of the spheroids are characterized.
More Related Videos
09:43Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
Published on: October 14, 2019
08:39High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately
Published on: July 8, 2014
Related Concept Videos
Precipitate Formation and Particle Size Control
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...
Base Quantities and Derived Quantities
The International Organization for...
Uniform Distribution
Two essential properties of this distribution are
Estimation of the Physical Quantities
Uniform Circular Motion
Non-uniform Circular Motion
For example, such...