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Related Experiment Video

Updated: Feb 11, 2026

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
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Assessing HER2 Amplification in Plasma cfDNA.

Isaac Garcia-Murillas1, Nicholas C Turner2,3

  • 1Breast Cancer Now Research Centre, The Institute of Cancer Research, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 3, 2018
PubMed
Summary
This summary is machine-generated.

Digital PCR (dPCR) offers precise DNA quantification by analyzing individual DNA portions. This study demonstrates dPCR

Keywords:
Breast cancerCirculating free DNADigital PCRHER2Plasma

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Digital PCR (dPCR) is a precise method for DNA concentration determination.
  • Circulating free DNA (cfDNA) analysis offers a noninvasive approach for molecular diagnostics.

Purpose of the Study:

  • To explore the application of dPCR for detecting oncogenic amplifications using cfDNA.
  • To develop a plasma cfDNA dPCR assay for HER2 copy number analysis.

Main Methods:

  • DNA partitioning into discrete single entities for individual analysis.
  • Development of a plasma cfDNA dPCR assay.
  • Quantification of HER2 copy number in cfDNA.

Main Results:

  • Demonstrated the feasibility of using dPCR for noninvasive oncogenic amplification detection.
  • Successfully developed and exemplified a plasma cfDNA dPCR assay for HER2 copy number.

Conclusions:

  • dPCR is a powerful tool for noninvasive oncogenic amplification detection via cfDNA.
  • The developed assay provides a sensitive method for HER2 copy number assessment.