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Related Concept Videos

Isothermal Processes01:21

Isothermal Processes

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A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes.
An ideal gas can also undergo isothermal expansion or compression.
For example, consider 1 mole of an ideal gas inside an isolated cylinder at initial volume V...
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Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

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Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
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Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

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Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
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Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

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Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
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Feedback Loops01:01

Feedback Loops

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In most cases, excessive hormone production is prevented by negative feedback—a loop that starts with a stimulus inducing the release of a particular substance, like a hormone, to maintain a certain level before triggering a signal that results in a decrease in further release of the hormone.
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Related Experiment Video

Updated: Feb 11, 2026

Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens
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Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens

Published on: February 20, 2015

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[Visualized detection for mycobacterium tuberculosis using loop-mediated isothermal amplification assay].

Na Zhao, Dianxing Sun, Jinxia Liu

    Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi
    |May 3, 2018
    PubMed
    Summary
    This summary is machine-generated.

    A new loop-mediated isothermal amplification (LAMP) assay with calcein visualization offers efficient and accurate detection of Mycobacterium tuberculosis (MTB). This method shows high sensitivity and specificity, making it suitable for resource-limited settings.

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    Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
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    Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

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    Reverse Transcription Loop-Mediated Isothermal Amplification RT-LAMP Assay for the Specific and Rapid Detection of Tilapia Lake Virus
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    Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
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    Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

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    Area of Science:

    • Microbiology
    • Molecular Diagnostics
    • Isothermal Amplification Techniques

    Background:

    • Tuberculosis remains a significant global health challenge, necessitating rapid and accurate diagnostic tools.
    • Existing diagnostic methods for Mycobacterium tuberculosis (MTB) can be slow or require specialized laboratory infrastructure.
    • Development of point-of-care diagnostic assays is crucial for effective tuberculosis control, especially in resource-limited environments.

    Purpose of the Study:

    • To establish and evaluate a loop-mediated isothermal amplification (LAMP) assay for the visualized detection of Mycobacterium tuberculosis (MTB).
    • To assess the sensitivity, specificity, and clinical practicability of the developed LAMP assay.
    • To compare the performance of the LAMP assay with the gold standard culture method.

    Main Methods:

    • Design of four LAMP primers targeting the 16S rRNA gene of MTB.
    • DNA extraction from clinical sputum samples.
    • Evaluation of assay specificity and sensitivity using standard MTB strains.
    • Visualized detection using calcein and comparison with electrophoresis.
    • Consistency evaluation with the culture method using Chi-squared test.

    Main Results:

    • The LAMP assay demonstrated high specificity with no non-specific amplification.
    • The detection limit of the assay was determined to be 10 copies/tube.
    • Visualized detection with calcein showed comparable sensitivity to electrophoresis.
    • Clinical evaluation yielded a sensitivity of 94.74% and specificity of 90%.
    • Statistical analysis (Chi-squared test) indicated good consistency between the LAMP assay and the culture method (P>0.05).

    Conclusions:

    • The established LAMP assay provides a highly efficient and visualized method for MTB detection.
    • The assay exhibits excellent sensitivity and specificity, comparable to the gold standard.
    • This technique holds significant promise for tuberculosis diagnosis in resource-limited settings, including field applications and primary care hospitals.