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Updated: Feb 11, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
A Carbapenem-Based Off-On Fluorescent Probe for Specific Detection of Metallo-β-Lactamase Activities
Wuyu Mao1, Yaqun Wang1, Xiana Qian1
1State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, P.R. China.
Abstract:
Metallo-β-lactamase is one of the major clinical threats because this β-lactam-hydrolyzing enzyme confers significant resistance to most β-lactam antibiotics, including carbapenems, among bacterial pathogens. Reported herein is a novel fluorogenic sensor for the specific detection of metallo-β-lactamase activities. This carbapenem-based reagent exhibits excellent selectivity to metallo-β-lactamase over other serine-β-lactamases, including serine carbapenemases. The usefulness of this probe was further demonstrated in the rapid identification of metallo-β-lactamase-expressing pathogenic bacteria.
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