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Related Experiment Video

Updated: Feb 11, 2026

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
06:40

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

Published on: April 1, 2021

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Smad4/DPC4.

Aoife J McCarthy1, Runjan Chetty1

  • 1Laboratory Medicine Program, Department of Anatomical Pathology, University Health Network and University of Toronto, Toronto, Ontario, Canada.

Journal of Clinical Pathology
|May 4, 2018
PubMed
Summary
This summary is machine-generated.

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See all related articles

Smad4 (also known as DPC4) is a tumor suppressor gene. Loss of Smad4 protein expression is a key indicator distinguishing pancreatic cancer from benign conditions in biopsies.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Smad4 (DPC4) is a crucial signal transducer in TGF-β pathways.
  • It functions as a tumor suppressor gene, with its inactivation implicated in various cancers.

Purpose of the Study:

  • To highlight the diagnostic utility of Smad4 protein expression in pancreatic cancer detection.
  • To review the role of Smad4 in different pathological conditions.

Main Methods:

  • Immunohistochemistry was employed to assess Smad4 protein localization and expression.
  • Analysis of Smad4 status in pancreatic cancer, juvenile polyposis syndrome, and other malignancies.

Main Results:

  • Smad4 protein is normally found in the cytoplasm and nucleus.
Keywords:
DPCTGF-βpancreatic cancersmad4tumour suppressor gene

Related Experiment Videos

Last Updated: Feb 11, 2026

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
06:40

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

Published on: April 1, 2021

5.4K
  • Loss of Smad4 expression is observed in high-grade pancreatic intraepithelial neoplasia and pancreatic cancer.
  • Smad4 inactivation is associated with pancreatic, colorectal, and prostate cancers, as well as juvenile polyposis syndrome.

    • Conclusions:

    • Immunohistochemistry for Smad4 is valuable for differentiating pancreatic cancer from benign conditions in biopsies.
    • Smad4 status is an important biomarker in various gastrointestinal and other cancers.