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MicroRNA-652 induces NED in LNCaP and EMT in PC3 prostate cancer cells

Robert K Nam1, Tania Benatar2, Yutaka Amemiya3

  • 1Division of Urology, Sunnybrook Health Sciences Centre, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada.

Oncotarget
|May 4, 2018
PubMed

Insights

High expression of microRNA-652 (miR-652) promotes prostate cancer progression and metastasis by targeting the tumor suppressor PP2A. This suggests miR-652 could be a biomarker for aggressive prostate cancer.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression.
  • miRNA dysregulation is linked to various diseases, including prostate cancer progression.
  • A panel of five miRNAs was identified, associated with prostate cancer recurrence and metastasis.

Purpose of the Study:

  • To investigate the role of miR-652 in prostate cancer progression.
  • To identify the molecular mechanisms by which miR-652 influences prostate cancer.
  • To evaluate miR-652 as a potential biomarker and therapeutic target for prostate cancer.

Main Methods:

  • Next-generation miRNA sequencing to identify key miRNAs.
  • Overexpression studies in prostate cancer cell lines (PC3, LNCaP).
  • Xenograft models to assess tumorigenicity and metastasis.
  • Analysis of target genes and downstream effects on cellular differentiation markers.

Main Results:

  • High miR-652 expression correlated with increased prostate cancer recurrence.
  • Overexpression of miR-652 enhanced prostate cancer cell growth, migration, invasion, tumorigenicity, and metastasis.
  • miR-652 directly targets PPP2R3A, reducing PP2A tumor suppressor function.
  • miR-652 induced epithelial-mesenchymal transition (EMT) in PC3 cells and neuroendocrine-like differentiation (NED) in LNCaP cells.

Conclusions:

  • miR-652 promotes prostate tumor progression by inducing EMT and NED through PP2A inhibition.
  • miR-652 expression is a potential biomarker for aggressive prostate cancer.
  • Targeting miR-652 offers a potential novel therapeutic strategy for prostate cancer.
Keywords:
EMTNEDmiR-652-3pmiRNAprostate cancer

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