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Acquisition of High-Quality Digital Video of Drosophila Larval and Adult Behaviors from a Lateral Perspective
Published on: October 4, 2014
Constant Quest for Quality: Digital Cytopathology
Simone L Van Es1,2,3, Janelle Greaves2, Stephanie Gay2
1Department of Pathology, School of Medical Sciences, The University of New South Wales, Sydney, NSW 2052, Australia.
Background:
Special consideration should be given when creating and selecting cytopathology specimens for digitization to maximize quality. Advances in scanning and viewing technology can also improve whole-slide imaging (WSI) output quality.
Methods:
Accumulated laboratory experience with digitization of glass cytopathology slides was collected.
Results:
This paper describes characteristics of a cytopathology glass slide that can reduce quality on resulting WSI. Important points in the glass cytopathology slide selection process, preparation, scanning, and WSI-editing process that will maximize the quality of the resulting acquired digital image are covered. The paper outlines scanning solutions which have potential to predict issues with a glass cytopathology slide before image acquisition, allowing for adjustment of the scanning approach. WSI viewing solutions that better simulate the traditional microscope experience are also discussed.
Conclusion:
In addition to taking advantage of technical advances, practical steps can taken to maximize quality of cytopathology WSI.
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