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Isolation and characterization of a group of new Proteus bacteriophages

V Morozova1, Yu Kozlova2, E Shedko2

  • 1Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentieva Ave. 8, Novosibirsk, 630090, Russia. morozova@niboch.nsc.ru.

Archives of Virology
|May 4, 2018
PubMed

Insights

New bacteriophages targeting multi-drug-resistant Proteus mirabilis were discovered. Three phages show promise for antibacterial therapy, while one represents a new genus, Pm16virus.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Multi-drug-resistant (MDR) Proteus mirabilis poses a significant threat to public health.
  • Bacteriophages offer a potential alternative to conventional antibiotics for combating MDR bacteria.

Purpose of the Study:

  • To isolate and characterize novel lytic bacteriophages active against MDR P. mirabilis strains.
  • To investigate the genomic and phylogenetic relationships of the isolated phages.

Main Methods:

  • Isolation of bacteriophages from cattle and poultry samples.
  • Electron microscopy for morphological classification.
  • Genome sequencing and comparative genomic analysis.
  • Phylogenetic analysis of essential viral proteins.

Main Results:

  • Four lytic bacteriophages (PM75, PM85, PM93, PM116) were isolated, active against MDR P. mirabilis.
  • All phages belong to the Podoviridae family.
  • Phages PM85, PM93, and PM116 show potential for therapeutic use.
  • Phages PM85, PM93, and PM116 are closely related to Proteus phage vB_PmiP_Pm5460.
  • Phage PM75 and PM16 are phylogenetically distinct, warranting classification as a new genus, "Pm16virus".

Conclusions:

  • The isolated bacteriophages are effective against MDR P. mirabilis.
  • Phages PM85, PM93, and PM116 are promising candidates for phage therapy.
  • Phage PM75 represents a novel genus, "Pm16virus", expanding our understanding of Proteus bacteriophages.

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