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Updated: Feb 11, 2026

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
[Galectin3 Regulates Transforming Growth Factor-β-induced Epithelial-mesenchymal]
Yong Qiang Wang1, Zi Hou Liu1, Jie Shu2
1Department of Cardiac Surgery, Central South University,Changsha 410008,China.
Galectin3 enhances transforming growth factor-beta (TGF-β)-induced epithelial-mesenchymal transition (EMT) in A549 lung cancer cells. This involves increased cell invasion and migration, highlighting Galectin3
Area of Science:
- Cell Biology
- Molecular Oncology
Background:
- Epithelial-mesenchymal transition (EMT) is crucial for cancer progression.
- Galectin3 is implicated in various cellular processes, including cancer.
Purpose of the Study:
- To investigate the role of Galectin3 in TGF-β-induced EMT in A549 lung cancer cells.
Main Methods:
- Overexpression of Galectin3 in A549 cells.
- Induction of EMT using TGF-β.
- Western blot and immunofluorescence for protein expression (Galectin3, E-cadherin, vimentin).
- Cell proliferation, migration, and invasion assays.
Main Results:
- Overexpressing Galectin3 alone did not alter EMT markers or cell behavior.
- TGF-β-induced EMT showed decreased E-cadherin and increased vimentin in Galectin3-overexpressing cells.
- Cellular invasion and migration were significantly enhanced in the presence of both TGF-β and Galectin3 overexpression.
Conclusions:
- Galectin3 potentiates TGF-β-induced EMT in A549 lung cancer cells.
- Galectin3 promotes enhanced cell invasion and migration during EMT.
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Published on: October 27, 2020
13:34High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition
Published on: June 1, 2019
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