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Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells
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Pyroptosis in pterygium pathogenesis.

Naiyu Sun1, Hong Zhang2

  • 1The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province, People's Republic of China.

Bioscience Reports
|May 5, 2018
PubMed
Summary

Pyroptosis, a programmed cell death linked to oxidative stress, plays a role in pterygium, an eye condition. Targeting pyroptosis may offer a new way to prevent pterygium development.

Keywords:
IL-1βNLRP3caspase-1oxidative stresspterygiumpyroptosis

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Immunology

Background:

  • Pterygium is an ocular disease involving fibrovascular tissue proliferation.
  • Oxidative stress is a key factor in pterygium development.
  • Pyroptosis, a programmed cell death pathway, is associated with oxidative stress.

Purpose of the Study:

  • To investigate the role of pyroptosis in the pathogenesis of pterygium.
  • To analyze the expression of pyroptosis-associated factors in pterygium tissues and cells.

Main Methods:

  • Analysis of NLRP3, caspase-1, IL-18, and IL-1β expression in human pterygium and conjunctival tissues using qRT-PCR and Western blot.
  • Primary culture of human conjunctival epithelial cells (HConECs) and human pterygium fibroblasts (HPFs).
  • Induction of pyroptosis in cultured cells using H₂O₂ and assessment of cell lysis, factor expression, and myofibroblast activation markers (α-SMA, E-cadherin).
  • Caspase-1 inhibition to confirm pyroptosis dependency.

Main Results:

  • Significantly increased expression of pyroptosis factors (NLRP3, caspase-1, IL-18, IL-1β) in pterygium tissues compared to normal conjunctiva.
  • H₂O₂-induced pyroptosis in HPFs and HConECs showed elevated pyroptosis markers.
  • Caspase-1 inhibition reduced IL-18 and IL-1β levels, confirming caspase-1-dependent pyroptosis.
  • Pyroptosis induction led to increased α-SMA and decreased E-cadherin in HPFs, which were reversed by caspase-1 inhibition.

Conclusions:

  • Pyroptosis is implicated in the pathological processes of pterygium formation and progression.
  • Caspase-1-dependent pyroptosis contributes to myofibroblast activation in pterygium.
  • Targeting pyroptosis presents a potential therapeutic strategy for preventing pterygium.