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Human prostasomes from normozoospermic and non-normozoospermic men show a differential protein expression pattern
A García-Rodríguez1, M de la Casa2, H Peinado3
1Biology Department, University Autónoma of Madrid, Madrid, Spain.
Andrology
|May 5, 2018
Summary
Prostasomes, vesicles from the prostate, contain proteins crucial for male fertility. Their protein content differs based on fertility status, suggesting potential as fertility biomarkers.
Area of Science:
- Reproductive Biology
- Proteomics
- Extracellular Vesicles
Background:
- Prostasomes are extracellular vesicles from prostatic epithelium, vital for male fertility.
- Their proteomic composition is known but its variation with fertility status is unclear.
Purpose of the Study:
- To investigate if prostasome protein content differs between normozoospermic and non-normozoospermic men.
- To identify potential protein biomarkers for male fertility status.
Main Methods:
- Isolation of prostasomes from seminal plasma of 12 normozoospermic and 14 non-normozoospermic men via differential ultracentrifugation.
- Quantitative mass spectrometry to analyze protein content.
- Comparative analysis of protein expression levels between the two groups.
Main Results:
- Identified 1282 proteins in prostasomes; 745 (57.8%) were common across samples.
- Found 47 differentially expressed proteins between the groups.
- Non-normozoospermic prostasomes showed underexpression of proteins involved in sperm energy metabolism and activity.
Conclusions:
- Prostasome protein content varies significantly with male fertility status.
- Differential protein expression in prostasomes correlates with fertility.
- Prostasomes show promise as potential biomarkers for assessing male reproductive health.
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