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Related Experiment Videos

Structure of the murine complement factor H gene.

D P Vik1, J B Keeney, P Muñoz-Cánoves

  • 1Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.

The Journal of Biological Chemistry
|November 15, 1988
PubMed
Summary
This summary is machine-generated.

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Factor H, a complement regulator, has a gene structure of 22 exons spanning 100 kb on chromosome 1. This genomic organization provides insight into the Factor H protein

Area of Science:

  • Genetics
  • Molecular Biology
  • Immunology

Background:

  • Factor H is a key regulator of the complement system's alternative pathway.
  • The protein consists of 20 tandem repeating units.

Purpose of the Study:

  • To analyze the genomic organization of the human Factor H gene.
  • To correlate gene structure with protein structure for functional studies.

Main Methods:

  • Identification of genomic clones using a Factor H cDNA.
  • Analysis of intron/exon junctions, 5' and 3' regions.
  • Mapping of exons within the Factor H gene.

Main Results:

  • The Factor H gene comprises 22 exons spanning approximately 100 kb on chromosome 1.

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  • Most repeating units are encoded by single exons, with exceptions for the second repeat and leader sequence.
  • Intron sizes vary, with the largest being the leader sequence exon's intron (26 kb).

    • Conclusions:

    • The genomic structure of the Factor H gene has been elucidated.
    • Understanding the gene's organization offers insights into Factor H protein structure.
    • This knowledge facilitates the creation of deletion mutants for future functional studies.