Related Experiment Video
Updated: Feb 11, 2026

Polysome Purification from Soybean Symbiotic Nodules
Published on: July 1, 2022
[Screening, resistance and growth-promoting effect of endophytic bacteria with ACC deaminase activity isolated from
Objective:
We screened endophytic bacteria containing ACC (1-aminocyclopropane-1-carboxylate) deaminase from soybean nodules, and evaluated salt-alkaline resistance, phylogenetic status and the growthpromoting of representative strain.
Methods:
The features of strains producing ACC deaminase were determined by using the ACC as a sole nitrogen source, adopting standard curve method, colorimetric method, solid medium screening method, bacterial morphology, physio-biochemical characteristics, similarity analysis of 16S rRNA gene, inoculation tests.
Results:
Eight endophytic bacteria containing ACC deaminase were screened from soybean nodules collected from 36 points of 13 cities (regions)in Henan province. Enzymaticactivity of DD132 was the highest (15.712 U/mg). Screened strain tolerated to medium of 4%-6% NaCl concentration. Among of them, DD165 and DD132 could tolerate 9% NaCl concentration. Five bacteria growing well under pH 11, showing that these strains had stronger alkali resistance. Eight strains containing ACC deaminase activity were affiliated to four genera: Bacillus, Enterobacter, Stenotrophomonas and Pantoea. Inoculation test showed that DD132 had a significant growthpromoting effect on wheat seedlings.
Conclusions:
Endophytic bacteria containing high ACC deaminase activity from soybean nodules have stronger salt-alkaline resistance. DD132 has obvious growth-promoting effect on wheat seedlings.
Related Concept Videos
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
The Roles of Bacteria and Fungi in Plant Nutrition
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Resistivity
Resistance

