Coexistence of NDM-1-producing Escherichia coli and Citrobacter freundii in the same patient

Although bla_NDM-1 has been widely detected in various Enterobacteriaceae clinical isolates, multiple bla_NDM-1 colonisations within the same patient remain rare. The aim of the study was to describe a patient with respiratory tract colonisation with NDM-1-producing Escherichia coli and Citrobacter freundii during hospitalisation in China.

Two carbapenem-resistant isolates were analysed. Antimicrobial susceptibility testing, metallo-β-lactamase production, conjugation assay, plasmid analysis and molecular typing were performed.

The two clinical isolates carried the bla_NDM-1 gene and showed resistance to carbapenems. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) revealed that E. coli NB753 harboured a plasmid of ca. 80kb, and C. freundii NB865 harboured three plasmids (ca. 70, 80 and 135kb). Southern blot and Inc replicon typing further demonstrated that both isolates carried the bla_NDM-1 gene on a self-transferrable IncX3 plasmid. Moreover, the two NDM-producing plasmids were conjugative and the transconjugants showed increased resistance to carbapenems.

An NDM-1-encoding plasmid harboured by various clinical isolates in a single patient is worrying considering that this plasmid may be widespread in our hospital. Furthermore, the threat of carbapenemase-producing bacterial epidemics should be closely monitored. However, a limitation of this study was the extremely small sample size.