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Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
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Enterodiol is Actively Transported by Rat Liver Cell Membranes
Alejandro de Athayde Moncorvo Collado1, Paula B Salazar1, Carlos Minahk2
1Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICETUNT, and Instituto de Química Biológica "Dr. Bernabé Bloj", Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI, San Miguel de Tucumán, Argentina.
The Journal of Membrane Biology
|May 6, 2018
Summary
Enterodiol interacts with liver membranes and is transported via ATP, unlike EGCG. This mechanism may explain enterodiol
Area of Science:
- Hepatology and Membrane Biology
- Molecular Pharmacology
- Nutraceuticals and Bioactive Compounds
Background:
- Epigallocatechin gallate (EGCG) interacts with phospholipid head groups but does not insert into membranes.
- Enterodiol shows no interaction with non-energized membranes.
- EGCG is extensively metabolized in the intestine and liver.
Purpose of the Study:
- To investigate the interaction and transport of enterodiol and EGCG with hepatic membranes.
- To elucidate the mechanism of enterodiol transport across liver membranes.
- To explain the higher blood concentrations of enterodiol compared to EGCG.
Main Methods:
- Incubation of hepatic membranes with enterodiol and EGCG.
- Measurement of ATPase activity in the presence of enterodiol and EGCG.
- Vesicle transport assays using liver membranes.
Main Results:
- Enterodiol interacts with and is transported by hepatic membranes upon ATP addition.
- Enterodiol significantly increases ATPase activity, while EGCG does not.
- EGCG binds to polar head groups but is not transported into membrane vesicles.
Conclusions:
- Hepatic membranes actively transport enterodiol, contributing to its systemic bioavailability.
- The observed transport mechanism may explain enterodiol's higher blood levels compared to EGCG.
- Enterodiol may evade hepatic inactivation through active efflux from hepatocytes.
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