Related Experiment Video

Updated: Feb 11, 2026

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
08:26

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Published on: August 23, 2022

2.9K

Loss of neuronal CD200 contributed to microglial activation after acute cerebral ischemia in mice

Yang Yang1, Xiang-Jian Zhang1, Cong Zhang1

  • 1Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, PR China; Hebei Key Laboratory of Vascular Homeostasis and Hebei Collaborative Innovation Center for Cardio-cerebrovascular Disease, 215 Hepingxi Road, Shijiazhuang, 050000, Hebei, PR China.

Neuroscience Letters
|May 6, 2018
PubMed

Insights

Loss of CD200 in neurons correlates with neuronal death after cerebral ischemia. Administering recombinant CD200 (rCD200) protein reduced microglial activation and inflammation in ischemic mice.

Area of Science:

  • Neuroscience
  • Immunology

Background:

  • CD200 plays a role in immuno-inflammatory responses.
  • Limited data exists on CD200's role in acute cerebral ischemia.

Purpose of the Study:

  • Investigate the association between CD200 expression and neuronal death in cerebral ischemia.
  • Explore the relationship between CD200 and microglia activation post-ischemia.

Main Methods:

  • Induced focal cerebral ischemia in mice (pMCAO).
  • Assessed CD200, neuron-specific enolase (NSE), and microglial activation (Iba-1) via Western blotting.
  • Analyzed neuronal cell death using flow cytometry and inflammatory cytokines (IL-1β, TNF-α, IL-10) via ELISA.
  • Administered intracerebroventricular recombinant CD200 (rCD200) protein post-ischemia.

Main Results:

  • CD200 is expressed in neurons, not microglia, in normal brain tissue.
  • CD200 and NSE expression decreased post-pMCAO, correlating negatively with neuronal death rate.
  • Exogenous rCD200 administration reduced microglial activation markers and inflammatory cytokines.

Conclusions:

  • CD200 loss is linked to neuronal death and contributes to microglial activation in cerebral ischemia.
  • Recombinant CD200 (rCD200) shows potential in suppressing microglial activation and inflammation in ischemic conditions.
Keywords:
CD200Cerebral ischemiaMicroglial activationNeuronal cell death

More Related Videos

Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion07:34

Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion

Published on: March 1, 2019

12.4K
Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice09:09

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Published on: August 9, 2013

41.0K

Related Concept Videos

Line Loss01:10

Line Loss

The different configurations of source-load connections include wye (star) and delta connections. The relationship between line and phase voltages and currents varies depending on the configuration. When the source is supplying power, it is transmitted through the wires to the load, and during this transmission, some power is absorbed by the wires, leading to line loss.
Line loss impacts power delivery efficiency in a balanced three-phase circuit. The symmetry in such a circuit simplifies the...
545
Binet's Contribution to Measures of Intelligence01:23

Binet's Contribution to Measures of Intelligence

Alfred Binet, along with his student Théophile Simon, was tasked by the French Ministry of Education in 1904 to create a method for identifying students who struggled to learn through conventional classroom instruction. This initiative aimed to address overcrowding by placing such students in specialized schools. Binet and Simon developed an intelligence test comprising 30 tasks, ranging from simple commands, like touching one's nose or ear, to more complex tasks, such as drawing...
1.8K
Reducing Line Loss01:18

Reducing Line Loss

In a three-phase circuit, line loss is an indicator of energy dissipated as heat due to the resistance of transmission lines. To address this, incorporating transformers into the system—a step-up transformer at the source and a step-down transformer at the load—is a strategic solution. Two three-phase transformers are introduced to improve this.
With a step-up transformer at the source, the voltage is increased, thereby reducing the current in the transmission lines since power loss in...
392
Wechsler's Contribution to Measures of Intelligence01:23

Wechsler's Contribution to Measures of Intelligence

David Wechsler, a psychologist who worked with World War I veterans, developed a significant IQ test in 1939 called the Wechsler-Bellevue Intelligence Scale. This test was innovative because it combined several subtests that measured both verbal and nonverbal skills, reflecting Wechsler's belief that intelligence is a global capacity involving purposeful action, rational thinking, and effective interaction with the environment. This test later evolved into the Wechsler Adult Intelligence...
2.1K
Major Losses in Pipes01:28

Major Losses in Pipes

When a fluid flows through a pipe, it experiences energy losses due to frictional resistance along the pipe walls, known as major losses. These energy losses result in a pressure drop, which varies based on the flow conditions — whether laminar or turbulent — and the specific physical properties of the fluid and pipe.
Fluid flow can be classified as laminar or turbulent, primarily based on the Reynolds number. This dimensionless number reflects the relative influence of inertial to viscous...
2.0K
Minor Losses in Pipes01:25

Minor Losses in Pipes

In pipe systems, minor losses refer to energy losses arising from components such as valves, bends, fittings, expansions, and other features that disrupt the steady flow of fluid. These disturbances cause energy dissipation through turbulence and resistance, which engineers quantify to manage system efficiency effectively.
Valves play a significant role in generating minor losses by obstructing or redirecting the fluid flow. When a valve is closed or partially closed, it restricts the flow...
2.0K