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A simple UV spectrophotometric method for theophylline serum level determination.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|September 15, 1978
Summary
A new UV spectrophotometric method offers a simple, inexpensive way to measure theophylline serum levels. This technique uses charcoal extraction for accurate theophylline isolation from biological fluids.
Area of Science:
- Analytical Chemistry
- Clinical Chemistry
Background:
- Theophylline is a critical medication requiring precise serum level monitoring.
- Existing methods for theophylline determination can be complex or costly.
Purpose of the Study:
- To develop a brief, simple, and inexpensive UV spectrophotometric method for determining theophylline serum levels.
- To validate the method's efficiency for clinical application.
Main Methods:
- The study employed UV spectrophotometry for quantitative analysis.
- Theophylline was isolated from biological fluids using charcoal extraction.
Main Results:
- The developed method is characterized by its brevity and simplicity.
- The method demonstrated comparable coefficients of variation and recovery rates to existing techniques.
- Charcoal extraction proved effective for isolating theophylline from biological samples.
Conclusions:
- The described UV spectrophotometric method is a viable, cost-effective alternative for routine theophylline serum level determination.
- The method's simplicity and comparable accuracy make it suitable for clinical laboratories.