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Identification of tick-borne pathogen diversity by metagenomic analysis in Haemaphysalis longicornis from Xinyang,

Lu Zhuang1,2,3, Juan Du1, Xiao-Ming Cui1

  • 1State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, Beijing, 100071, People's Republic of China.

Infectious Diseases of Poverty
|May 8, 2018
PubMed
Abstract

Insights

Haemaphysalis longicornis ticks in China carry diverse pathogens, including Anaplasma, Rickettsia, Babesia, and SFTSV. This highlights significant risks of tick-borne diseases in the region.

Area of Science:

  • Veterinary Entomology
  • Molecular Biology
  • Public Health

Background:

  • Haemaphysalis longicornis is a significant vector for various pathogens.
  • Tick-borne diseases pose a considerable threat in endemic areas.
  • Understanding pathogen carriage in vectors is crucial for disease prevention.

Purpose of the Study:

  • To systematically identify pathogens harbored by Haemaphysalis longicornis in a tick-borne disease epidemic area in China.
  • To assess the potential risk of human infection from tick bites in this region.

Main Methods:

  • Adult Haemaphysalis longicornis ticks were collected in Xinyang, central China.
  • High-throughput sequencing was used to analyze genomic DNA and RNA from individual ticks.
  • Specific PCR assays and phylogenetic analysis were employed for pathogen confirmation and evolutionary study.

Main Results:

  • Anaplasma spp. (A. bovis, A. centrale), Rickettsia spp. (R. heilongjiangensis, R. sp. LON-13, R. raoultii), and Babesia sp. were detected.
  • Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) was the most prevalent pathogen (18.75%).
  • Co-infection of SFTSV and Anaplasma bovis was observed in 2.08% of ticks.

Conclusions:

  • Haemaphysalis longicornis harbors a wide array of prokaryotic and eukaryotic microorganisms.
  • Infection with Anaplasma spp., Rickettsiae spp., Babesia spp., and SFTSV should be considered in patients with a history of tick bites in this region.
  • A novel Babesia species was identified, warranting further investigation.
Keywords:
Haemaphysalis longicornisHigh-throughput sequencingPathogens

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