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Related Concept Videos

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A schema is a mental framework that helps individuals organize and interpret information. Schemata, formed from previous experiences, influence how we process new information: how we encode it, the inferences we make, and how we retrieve it. For instance, a schema for what a typical classroom looks like might include desks, a teacher's desk, a whiteboard, and students in such an environment. This expectation helps us quickly understand and navigate new classrooms without needing to analyze...
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Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
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ATP is a highly unstable molecule. Unless quickly used to perform work, ATP spontaneously dissociates into ADP and inorganic phosphate (Pi), and the free energy released during this process is lost as heat. The energy released by ATP hydrolysis is used to perform work inside the cell and depends on a strategy called energy coupling. Cells couple the exergonic reaction of ATP hydrolysis with endergonic reactions, allowing them to proceed.
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Sugar (a simple carbohydrate) metabolism (chemical reactions) is a classic example of the many cellular processes that use and produce energy. Living things consume sugar as a major energy source because sugar molecules have considerable energy stored within their bonds. Consumed carbohydrates have their origins in photosynthesizing organisms like plants. During photosynthesis, plants use the energy of sunlight to convert carbon dioxide gas into sugar molecules, like glucose. Because this...
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Triglycerides are a form of long-term energy storage molecules. They are made of glycerol and three fatty acids. To obtain energy from fat, triglycerides must first be broken down by hydrolysis into their two principal components, fatty acids and glycerol. This process, called lipolysis, takes place in the cytoplasm. The resulting fatty acids are oxidized by β-oxidation into acetyl-CoA, which is used by the Krebs cycle. The glycerol that is released from triglycerides after lipolysis...
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Related Experiment Video

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Liver Cold Storage and Transplantation in the Cold-Adaptive Daurian Ground Squirrels
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Supercooling Storage for the Transplantable Sources From the Rat and the Rabbit: A Preliminary Report.

T Sultana1, J I Lee2, J H Park3

  • 1Regenerative Medicine Laboratory, Center for Stem Cell Research, Department of Biomedical Science and Technology, Institute of Biomedical Science and Technology, Seoul, Republic of Korea.

Transplantation Proceedings
|May 8, 2018
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Summary
This summary is machine-generated.

Supercooling, a method of cooling below freezing without ice formation, shows promise for preserving transplantable cells and organs. This technique slowed the decrease in mesenchymal stem cell viability and minimized damage to rat kidneys compared to other storage methods.

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Area of Science:

  • Biopreservation
  • Cryobiology
  • Transplantation Science

Background:

  • Effective preservation of transplantable cells and organs is critical for various manipulation and processing steps.
  • Preservation methods, influenced by factors like temperature and solvent, impact sample safety and efficacy.
  • Supercooling, a state below freezing point without ice formation, presents a potential preservation strategy.

Purpose of the Study:

  • To investigate the feasibility of supercooling for preserving cells and organs under controlled conditions.
  • To evaluate the impact of supercooling on the viability of mesenchymal stem cells (MSCs) and the histopathological integrity of neonatal rat kidneys.

Main Methods:

  • Mesenchymal stem cells (MSCs) from rabbits and neonatal rat kidneys were preserved in histidine-tryptophan-ketoglutarate solution.
  • Samples were stored for 48 hours at various temperatures, including supercooling conditions (-2 °C and -5 °C).
  • Viability of MSCs and histopathological changes in kidneys were assessed and compared across temperature groups.

Main Results:

  • MSCs showed a slower decrease in viability under cooling and supercooling conditions compared to room temperature storage.
  • Rat kidneys stored at -2 °C under supercooling exhibited minimal damage to the cortex and medulla parenchyma.
  • Supercooling at -2 °C appeared to offer superior preservation for neonatal rat kidneys.

Conclusions:

  • Storage temperature significantly affects the integrity and viability of transplantable sources.
  • Supercooling demonstrates potential as an effective preservation method for cells, tissues, and organs.
  • Further research is needed to optimize supercooling temperatures for diverse biological samples.