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MicroRNA-192-5p suppresses the initiation and progression of osteosarcoma by targeting USP1

Sheng Zhou1, Min Xiong2, Guo Dai1

  • 1Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

Oncology Letters
|May 8, 2018
PubMed

Insights

MicroRNAs (miRNAs) regulate osteosarcoma. This study shows miR-192-5p inhibits osteosarcoma progression by targeting USP1, suggesting potential diagnostic and therapeutic applications for this miRNA and its pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is the most common primary bone malignancy.
  • MicroRNAs (miRNAs) are key regulators in osteosarcoma development and progression.

Purpose of the Study:

  • To investigate the function of miR-192-5p and ubiquitin-specific protease 1 (USP1) in osteosarcoma.
  • To determine if miR-192-5p directly targets USP1 in osteosarcoma.

Main Methods:

  • Expression analysis of miR-192-5p and USP1 in osteosarcoma tissues and cell lines.
  • Functional assays assessing the impact of miR-192-5p and USP1 on cell proliferation, apoptosis, migration, and invasion.
  • Investigation of the direct interaction between miR-192-5p and USP1.

Main Results:

  • miR-192-5p was significantly downregulated in osteosarcoma, with USP1 showing an inverse expression.
  • miR-192-5p overexpression suppressed osteosarcoma cell proliferation, migration, and invasion, while enhancing cisplatin sensitivity.
  • USP1 was identified as a direct target of miR-192-5p; USP1 overexpression promoted tumor progression and reduced chemo-sensitivity, effects partially reversed by miR-192-5p.

Conclusions:

  • miR-192-5p suppresses osteosarcoma initiation and progression by targeting USP1.
  • miR-192-5p may serve as a valuable biomarker for osteosarcoma.
  • The miR-192-5p/USP1 axis represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
microRNAmicroRNA-192-5posteosarcomatargeted therapyubiquitin-specific protease 1

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