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Related Experiment Video

Updated: Feb 11, 2026

Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
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Label-free and live cell imaging by interferometric scattering microscopy.

Jin-Sung Park1, Il-Buem Lee1,2, Hyeon-Min Moon1,2

  • 1Center for Molecular Spectroscopy and Dynamics , Institute for Basic Science , Seoul 02841 , Korea.

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|May 8, 2018
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Summary

Label-free interferometric scattering microscopy enables live-cell imaging of cellular structures without fluorescent labels. This technique visualizes cytoplasmic organelles and cell-substrate contacts in various cell types.

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Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • Observing intracellular structures in live cells typically requires fluorescent labeling, which can perturb cellular functions.
  • Existing high-resolution microscopy techniques often face limitations in label-free live-cell imaging of complex organelles.

Purpose of the Study:

  • To develop and demonstrate a label-free microscopy method for visualizing cytoplasmic organelles and cell structures in live cells.
  • To assess the capability of interferometric scattering microscopy for imaging diverse cell types and their subcellular components.

Main Methods:

  • Utilized interferometric scattering microscopy (iSCAT) for live-cell imaging.
  • Applied the technique to mammalian cells, *Escherichia coli*, and yeast.
  • Analyzed images to identify cytoplasmic organelles and cell-substrate contact sites.

Main Results:

  • Achieved label-free, live-cell imaging of mammalian cells, *E. coli*, and yeast.
  • Successfully visualized various cytoplasmic organelles and the underside structures of cells.
  • Clearly discerned cell-substrate contact areas, including focal adhesions and filopodia.
  • Observed fringe-like features potentially representing folded organelle structures.

Conclusions:

  • Interferometric scattering microscopy provides a powerful label-free approach for live-cell imaging.
  • The technique offers insights into the in vivo structures and dynamics of intracellular phenomena.
  • This method holds promise for advancing the study of cellular biology without the need for exogenous labels.