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Updated: Feb 11, 2026

An Operant Intra-/Extra-dimensional Set-shift Task for Mice
Published on: January 22, 2016
A red-shifted two-photon-only caging group for three-dimensional photorelease
Yvonne Becker1, Erik Unger1, Manuela A H Fichte1
1Goethe University Frankfurt , Institute for Organic Chemistry and Chemical Biology , Max-von-Laue-Str. 7 , 60438 Frankfurt , Germany .
Abstract:
Based on nitrodibenzofuran (NDBF) a new photocage with higher two-photon action cross section and red-shifted absorption was developed. Due to calculations, a dimethylamino functionality (DMA) was added at ring position 7. The uncaging of nucleobases after two-photon excitation (2PE) could be visualized via double-strand displacement in a hydrogel. With this assay we achieved three-dimensional photorelease of DMA-NDBF-protected DNA orthogonal to NDBF-protected strands. While being an excellent 2P-cage, DMA-NDBF is surprisingly stable under visible-light one-photon excitation (1PE). This case of excitation-specific photochemistry enhances the scope of orthogonal photoregulation.
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