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Spironaphthoxazine switchable dyes for biological imaging.

Yaoyao Xiong1, Andreas Vargas Jentzsch1, Johannes W M Osterrieth1

  • 1Department of Chemistry , University of Oxford , Chemistry Research Laboratory , Oxford OX1 3TA , UK .

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We developed a novel spironaphthoxazine photochromic switch for super-resolution microscopy. This molecular switch exhibits high photoconversion, fatigue resistance, and fast switching speeds, enabling advanced cellular imaging applications.

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Area of Science:

  • Organic Chemistry
  • Photochemistry
  • Microscopy

Background:

  • Super-resolution microscopy demands advanced switchable dyes.
  • Molecular switches are crucial for developing new imaging tools.

Purpose of the Study:

  • To synthesize and characterize a novel spironaphthoxazine photochromic switch.
  • To evaluate its performance for applications in RESOLFT microscopy.

Main Methods:

  • Synthesis and characterization of a spironaphthoxazine derivative.
  • Photophysical studies including time-resolved infrared and transient absorption spectroscopy.
  • Evaluation of photochromic properties like photoconversion, fatigue resistance, and switching kinetics.

Main Results:

  • High photoconversion (85-95%) achieved with 405 nm light.
  • Excellent fatigue resistance and broad visible absorption.
  • Fast ring-opening (2.1 ns) and ring-closing (0.28 ns) kinetics.
  • Successful demonstration in switchable fluorescent dyads for live-cell imaging.

Conclusions:

  • The developed spironaphthoxazine is a highly efficient photochromic switch.
  • Its properties are suitable for advanced super-resolution microscopy techniques like RESOLFT.
  • This molecular switch enables high-contrast, fatigue-resistant live-cell imaging.