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An Enzyme-Responsive "Turn-on" Fluorescence Polymeric Superamphiphile as a Potential Visualizable Phosphate Prodrug
Xi Yang1, Shihong Shen2, Li Guo3
1Department of Applied Chemistry, School of Science, MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.
Macromolecular Bioscience
|May 8, 2018
Summary
This study introduces a novel fluorescent superamphiphile for drug delivery. Enzyme-responsive polymer disintegration triggers a "turn-on" fluorescence, enabling real-time sensing.
Area of Science:
- Polymer Chemistry
- Biomaterials Science
- Nanotechnology
Background:
- Enzyme-responsive polymers are crucial for advanced drug delivery systems.
- Developing efficient and inexpensive materials is key for targeted therapies.
- Fluorescent sensing offers real-time monitoring capabilities.
Purpose of the Study:
- To design a novel superamphiphile capable of fluorescent dissociation sensing.
- To utilize enzyme-responsive polymer disintegration for drug delivery monitoring.
- To create a "turn-on" fluorescence system for real-time detection.
Main Methods:
- Synthesis of a cationic diblock copolymer: methoxy-poly(ethylene glycol)113 -b-poly(2-dimethyl-aminoethyl methacrylate)70 .
- Assembly of the superamphiphile using adenosine 5 omino-triphosphate (ATP) and fluorescein diphosphate (FDP).
- Enzymatic hydrolysis triggered by calf intestinal alkaline phosphatase to induce superamphiphile disintegration and fluorescence.
- In vitro application testing of the superamphiphile system.
Main Results:
- The superamphiphile successfully disintegrated upon exposure to calf intestinal alkaline phosphatase.
- Enzymatic hydrolysis of ATP was presumed to cause superamphiphile breakdown.
- Fluorescein diphosphate hydrolysis led to a significant increase in fluorescence emission.
- The system demonstrated effective in vitro applications for sensing disintegration.
Conclusions:
- A novel fluorescent superamphiphile was successfully designed and synthesized.
- The "turn-on" fluorescence mechanism provides a reliable method for real-time detection of polymer disintegration.
- This enzyme-responsive system holds promise for targeted drug delivery applications.