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Updated: Feb 11, 2026

Microbial Communities in Nature and Laboratory - Interview
Published on: May 28, 2007
Microbial transformation of pseudoprotodioscin by Gibberella fujikuroi
Hong-Xiu Hu1, Ran-Ran Gao1, Zhao-Hui Gao1
1a Natural Medicine Chemistry Research Center, Institute of Medicinal Plant Development , Chinese Academy of Medical Sciences & Peking Union Medical College , Beijing 100193 , China.
Abstract:
Three new (6, 9, and 12) and nine known steroidal saponins were obtained from the fermentation broth of pseudoprotodioscin (PPD) incubated with a fungus Gibberella fujikuroi CGMCC 3.4663. Structures of the metabolites were elucidated by 1-D (1H, 13C), 2-D (HMBC, HSQC, NOESY) NMR, and HR-MS analyses. The biotransformation pathway of pseudoprotodioscin by Gibberella fujikuroi CGMCC 3.4663 was proposed. Compounds 1-11 were tested in vitro for their cytotoxic activities against two human cancer cell lines (HepG2 and Hela). Compounds 1, 6, 9, and 10 exhibited cytotoxic activity against HepG2 cells. Compound 10 exhibited cytotoxicity to Hela cells.
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