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Updated: Feb 11, 2026

Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays
Published on: October 1, 2013
[Effects of different microbial agents on substrate enzyme activities and tomato yield and quality.]
Le Mei Hou1, Rui Qing Meng1, Lan Chun Nie1
1College of Horticulture, Agricultural University of Hebei, Baoding 071000, Hebei, China.
Abstract:
Chicken manure substrate (fermented chicken manure:fermented corn stalk:river sand=3:4:3) and cattle manure substrate (fermented cattle manure:fermented corn stalk:river sand=3:4:3) were used to study the effects of microbial agents ('Difulai', enzyme microorganism, EM bacteria, Bacillus subtilis, and agricultural microbial agent) on substrate enzyme activities and tomato yield and quality. The results showed that the activities of urease, invertase and alkaline phosphatase in both two substrates all significantly increased at 40 d and 60 d after microbial agent addition. The growth, yield and vitamin C content of tomato were higher than those of control. Among the microbial agents, 'Difulai' showed the best effect in both two substrates. Plant yield increased by 14.7% and 40.0%, tomato vitamin C content increased by 22.2% and 39.7% respectively in chicken manure and cattle manure substrate with 'Difulai' addition. Yield and vitamin C content of tomato plant in chicken manure substrate without microbial agent addition were higher than that in cattle manure substrate, while there was no significant difference in two substrates with 'Difulai' addition.
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