Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Visual colorimetric 'turn-off' biosensor for ascorbic acid detection based on

Chunlei Mu1, Haifeng Lu1, Jie Bao2

  • 1Anhui No. 2 Province People's Hospital, Hefei 200041, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|May 8, 2018
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cyanidin-3-O-arabinoside improves high-fat diet-induced glucose and lipid metabolism disorder in mice by restoring fatty acid oxidation and mitochondrial function through CD36/AMPK/PGC-1α.

General physiology and biophysics·2026
Same author

Lysine acetyltransferase 8-mediated histone acetylation, regulated by GBA1, is associated with lysosomal function related to α-Synuclein pathology.

Cell death & disease·2026
Same author

Clinical Significance of B7-H4 in Peripheral Blood of Patients With Myasthenia Gravis.

Journal of immunology research·2026
Same author

Tracking ClO<sup>-</sup> Dynamics during the Progression of Parkinson's Disease in MPTP Mouse Model Using <i>In Vivo</i> Brain Microdialysis.

ACS chemical neuroscience·2026
Same author

Design of fault water-resisting coal pillars based on deep-beam analysis: A comparison of two analytical methods.

PloS one·2026
Same author

Multicohort Validation of Gut Microbiome Signatures for Cholangiocarcinoma Diagnosis and Functional Characterization of Bifidobacterium Pseudocatenulatum.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Benzothiadiazole-based covalent organic frameworks with red-light emission for visual detection of norfloxacin.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Photophysical behavior of mono- and bis(2'-hydroxyphenyl)-6-(4'-diphenylaminophenyl)pyrimidines: Interplay of ESIPT, ICT, and vibrational nonradiative decay.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Lanthanide-based metal-organic gels for flexible X-ray imaging.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Non-destructive identification of mineral pigments in painted cultural heritage based on hyperspectral imaging technology.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Dual-DNA nanoball-mediated 3D catalytic hairpin assembly for in situ imaging of miRNA-155 in living cells.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same journal

Turn-on fluorescent assay based on nitrogen-doped carbon dots and aptamer for low-background detection of kanamycin.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
See all related articles

A new colorimetric method detects ascorbic acid (AA) by observing the fading of a blue color. This simple, rapid assay accurately measures AA in orange juice using UV-vis spectroscopy.

Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Ascorbic acid (AA) is a vital nutrient with numerous biological roles.
  • Accurate quantification of AA is crucial in food analysis and biomedical research.
  • Existing methods for AA detection can be complex or require specialized equipment.

Purpose of the Study:

  • To develop a sensitive and cost-effective colorimetric assay for ascorbic acid (AA) detection.
  • To utilize the reducing properties of AA for a 'turn-off' sensing mechanism.
  • To validate the method for real-world sample analysis, such as in fruit juices.

Main Methods:

  • A colorimetric probe, 3,3',5,5'-tetramethylbenzidine (TMB), was employed.
  • Hypochlorite (ClO-) oxidized TMB to a blue-colored product (oxTMB).
  • Ascorbic acid reduced oxTMB, causing a decrease in absorbance at 652 nm, forming the basis of the 'turn-off' assay.
Keywords:
3,3′,5,5′,-TetramethylbenzidineAscorbic acidColorimetricHypochlorite‘Turn–off’ biosensor

Related Experiment Videos

Main Results:

  • The assay demonstrated a good linear response for AA concentrations from 1 to 70 μM.
  • A low detection limit of 0.58 μM was achieved.
  • The method successfully quantified AA in orange juice with recoveries exceeding 97.0% and showed good agreement with HPLC analysis (3.3% average relative error).

Conclusions:

  • A simple, rapid, and low-cost colorimetric method for AA determination using UV-vis spectroscopy was successfully developed.
  • The assay does not require nanomaterials, making it accessible and practical.
  • The method's effectiveness in analyzing real samples like orange juice highlights its potential for routine analysis.