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Updated: Feb 11, 2026
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Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
Published on: May 27, 2015
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Endoglin is Highly Expressed in Human Mast Cells
Suéllen Trentin Brum1, Ana Paula Demasi1, Rafael Fantelli Stelini2
1Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center.
Summary
Endoglin is a reliable biomarker for identifying mast cells, comparable to standard stains. This finding aids in diagnosing mast cell-related conditions and understanding their role in diseases like cancer.
Area of Science:
- Immunohistochemistry
- Oncology
- Cell Biology
Background:
- Endoglin is expressed in proliferating vessels and is a prognostic factor in malignancies.
- Endoglin is associated with adverse outcomes in childhood acute lymphoblastic leukemia.
- Mast cells regulate inflammation and neoplasia.
Purpose of the Study:
- To evaluate endoglin as a biological marker for mast cells.
- To compare endoglin with gold standard mast cell stains.
Main Methods:
- Immunohistochemistry for endoglin and mast cell tryptase.
- Histochemical staining using toluidine blue.
- Quantitative analysis of 15 neurofibroma, 9 mastocytosis, and 6 fibrous scar tissue specimens.
Main Results:
- Strong endoglin expression was observed in mast cell cytoplasmic granules across all studied groups.
- Endoglin showed similar results to mast cell tryptase and toluidine blue.
- Intraclass correlation coefficient confirmed endoglin as a highly reliable mast cell biomarker.
Conclusions:
- Endoglin can assist in diagnosing and studying the pathogenesis of mast cell-associated processes.
- Endoglin-neutralizing treatments may impact mastocytes and the immune system in cancers.
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