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Updated: Feb 11, 2026
Transoral Robotic Total Thyroidectomy and Bilateral Central Regional Lymph Node Dissection for Papillary Thyroid Carcinoma
Published on: September 15, 2023
Long Noncoding RNA AB074169 Inhibits Cell Proliferation via Modulation of KHSRP-Mediated CDKN1a Expression in
Qiheng Gou1,2, Linbo Gao3, Xinwen Nie4
1Department of Thoracic Surgery, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Collaborative Innovation Center of Biotherapy, Chengdu, China.
Abstract:
Long noncoding RNAs (lncRNA) are emerging as a novel class of regulators in gene expression associated with tumorigenesis. However, the role of lncRNAs in papillary thyroid carcinoma (PTC) is poorly understood. Here, we conducted global lncRNA profiling and identified lncRNA AB074169 (lncAB) as significantly downregulated in PTC. Decreased expression of lncAB in PTC was caused by CpG hypermethylation within its gene promoter. Functional studies showed that lncAB overexpression led to cell-cycle arrest and tumor growth inhibition in vitro and in vivo, whereas lncAB knockdown promoted cell proliferation. Mechanistic analyses revealed that lncAB bound KH-type splicing regulatory protein (KHSRP) and also decreased expression of KHSRP, thus increasing CDKN1a (p21) expression and decreasing CDK2 expression to repress cell proliferation. Taken together, these findings demonstrate that lncAB functions as a tumor suppressor during PTC tumorigenesis.Significance: These findings identify a tumor-suppressive long noncoding RNA in papillary thyroid carcinoma.Graphical Abstract: http://cancerres.aacrjournals.org/content/canres/78/15/4163/F1.large.jpg Cancer Res; 78(15); 4163-74. ©2018 AACR.
Insights
Long noncoding RNA AB074169 (lncAB) acts as a tumor suppressor in papillary thyroid carcinoma (PTC). Its downregulation, due to promoter hypermethylation, promotes PTC cell proliferation and tumor growth.
Area of Science:
- Molecular Biology
- Cancer Research
- Genetics
Background:
- Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in gene regulation and cancer development.
- The specific functions of lncRNAs in papillary thyroid carcinoma (PTC) remain largely unexplored.
Purpose of the Study:
- To investigate the role of lncRNAs in papillary thyroid carcinoma (PTC) pathogenesis.
- To identify and characterize novel lncRNAs involved in PTC development.
Main Methods:
- Global lncRNA profiling was performed to identify differentially expressed lncRNAs in PTC.
- Functional studies, including overexpression and knockdown experiments, were conducted in vitro and in vivo.
- Mechanistic investigations involved analyzing the interaction of lncAB with KH-type splicing regulatory protein (KHSRP) and its downstream effects on cell cycle regulators.
Main Results:
- lncRNA AB074169 (lncAB) was found to be significantly downregulated in PTC tissues.
- CpG hypermethylation of the lncAB promoter was identified as the cause of its decreased expression.
- lncAB overexpression inhibited PTC cell proliferation and tumor growth, while lncAB knockdown promoted proliferation.
- lncAB was shown to bind KHSRP, leading to decreased KHSRP expression, increased CDKN1a (p21) expression, and decreased CDK2 expression, ultimately repressing cell proliferation.
Conclusions:
- lncAB functions as a tumor suppressor in papillary thyroid carcinoma (PTC).
- The findings identify lncAB as a potential therapeutic target for PTC.
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