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A Systematic Approach to the Selection of the Appropriate Avibactam Concentration for Use with Ceftazidime in Broth
Patricia A Bradford1, Michael D Huband1, Gregory G Stone2
1AstraZeneca Pharmaceuticals, Waltham, Massachussetts, USA.
Abstract:
Selection of the avibactam concentration to combine with ceftazidime in susceptibility testing was determined using Gram-negative isolates with characterized β-lactamases predefined as susceptible or resistant, based on the known inhibition spectrum of avibactam. MIC values were determined by broth microdilution of ceftazidime with fixed concentrations and ratios of avibactam. A constant concentration of 4 μg/ml of avibactam was selected for susceptibility testing with ceftazidime because of its ability to correctly categorize susceptible and resistant isolates while minimizing categorical errors.
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