Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interaction at a distance between lambda repressors disrupts gene activation.

A Hochschild1, M Ptashne

  • 1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.

Nature
|November 24, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme.

Proceedings of the National Academy of Sciences of the United States of America·2004
Same author

Transcription initiation: imposing specificity by localization.

Essays in biochemistry·2002
Same author

Bacterial two-hybrid analysis of interactions between region 4 of the sigma(70) subunit of RNA polymerase and the transcriptional regulators Rsd from Escherichia coli and AlgQ from Pseudomonas aeruginosa.

Journal of bacteriology·2001
Same author

A chimeric activator of transcription that uses two DNA-binding domains to make simultaneous contact with pairs of recognition sites.

Molecular microbiology·2001
Same author

Towards a minimal motif for artificial transcriptional activators.

Chemistry & biology·2001
Same author

Telomere looping permits gene activation by a downstream UAS in yeast.

Nature·2001
Same journal

Harmonizing standards and resources for the medical genome.

Nature·2026
Same journal

Towards the construction of a virtual yeast.

Nature·2026
Same journal

Aerosols and hydrocarbons in the atmosphere of a white dwarf planet.

Nature·2026
Same journal

TROP2 targeting reveals therapy-driven cell state dynamics in colorectal cancer.

Nature·2026
Same journal

Competing programs shape cortical sensorimotor-association axis development.

Nature·2026
Same journal

Steatosis shapes prognosis-defining liver metastasis heterogeneity in CRC.

Nature·2026
See all related articles

The lambda repressor protein controls gene activity by both activating and repressing transcription. Its activation function is blocked by another repressor molecule, forming a DNA loop for negative control.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The lambda repressor protein plays a dual role in gene regulation.
  • Understanding its regulatory mechanisms is crucial for comprehending transcriptional control.

Purpose of the Study:

  • To elucidate the mechanism by which the lambda repressor's activation function is inhibited.
  • To investigate the role of DNA looping in transcriptional regulation.

Main Methods:

  • Biochemical assays to study protein-DNA interactions.
  • Analysis of transcriptional activity in vitro and in vivo.
  • Structural studies of DNA-protein complexes.

Main Results:

  • The lambda repressor acts as both an activator and repressor of transcription.

Related Experiment Videos

  • Interaction with an upstream repressor molecule blocks the repressor's activation function.
  • Formation of a DNA loop facilitates this long-range negative control.

    • Conclusions:

    • The study demonstrates a novel mechanism of transcriptional regulation mediated by DNA looping.
    • This finding provides insights into how regulatory proteins can control gene expression from a distance.