Related Experiment Video

Updated: Feb 10, 2026

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers
11:34

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

Published on: December 5, 2017

13.1K

LMO4 mediates trastuzumab resistance in HER2 positive breast cancer cells

Keshuo Ding1, Zhengsheng Wu1, Xiaocan Li2

  • 1Department of Pathology, Anhui Medical University Hefei, Anhui, P. R. China.

American Journal of Cancer Research
|May 9, 2018
PubMed

Insights

LMO4 promotes trastuzumab resistance in HER2 positive breast cancer, hindering treatment effectiveness. Targeting LMO4 may offer new diagnostic and therapeutic strategies for breast cancer patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Breast cancer is a leading cause of cancer mortality in women globally.
  • Trastuzumab is effective for HER2 positive breast cancer, but resistance limits its use.
  • Understanding resistance mechanisms is crucial for improving breast cancer treatment outcomes.

Purpose of the Study:

  • To investigate the role of LMO4 in trastuzumab resistance in HER2 positive breast cancer.
  • To explore LMO4 as a potential therapeutic target for overcoming trastuzumab resistance.

Main Methods:

  • Over-expression and depletion of LMO4 in human breast cancer cell lines (SKBR3 HR, BT474 HR).
  • In vitro and in vivo experiments to assess trastuzumab resistance.
  • Analysis of BCL-2 regulation by LMO4.
  • Correlation analysis of LMO4 levels with clinicopathological parameters and patient survival.

Main Results:

  • LMO4 is over-expressed in acquired trastuzumab-resistant breast cancer cells.
  • LMO4 depletion partially reversed trastuzumab resistance.
  • Forced LMO4 expression increased trastuzumab resistance both in vitro and in vivo.
  • LMO4 regulates BCL-2, mediating its role in resistance.
  • High LMO4 levels correlate with adverse clinicopathological features and reduced survival in HER2 positive breast cancer patients.

Conclusions:

  • LMO4 promotes trastuzumab resistance in HER2 positive breast cancer by regulating BCL-2.
  • LMO4 is a potential biomarker for predicting treatment response and prognosis.
  • Targeting LMO4 presents a promising strategy for overcoming trastuzumab resistance in breast cancer.
Keywords:
BCL-2HER2LMO4breast cancertrastuzumab resistance

More Related Videos

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers06:02

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers

Published on: February 20, 2017

8.0K
Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model09:29

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

18.9K

Related Concept Videos

Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.8K
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
7.1K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.6K
Resistance01:19

Resistance

When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
6.0K
Serial Position Effect01:03

Serial Position Effect

The serial position effect is a cognitive phenomenon where individuals are more likely to recall the first and last items in a list compared to those in the middle. This effect is divided into the primacy effect and the recency effect. The primacy effect is observed when the initial items in a list are remembered better. This occurs because these items are rehearsed more frequently or receive more elaborative processing, allowing them to be encoded into long-term memory more effectively. For...
563