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[Adjusting Platelet Counts for Platelet Aggregation Tests].

Li-Qin Ling1,2, Xin-Chun Yang3, Hao Chen1,2

  • 1Department of Laboratory Medicine,West China Hospital,Sichuan University,Chengdu 610041,China.

Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition
|May 9, 2018
PubMed
Summary

Physiological saline (PS) is recommended for adjusting platelet counts in light transmission aggregometry (LTA) for most agonists. Whole blood-derived platelet-poor plasma (PPP) offers convenience for ristocetin-induced aggregation.

Keywords:
Physiological salinePlatelet aggregationPlatelet-poor plasmaPlatelet-rich plasma

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Area of Science:

  • Hematology
  • Clinical Chemistry
  • Platelet Function Testing

Background:

  • Light transmission aggregometry (LTA) is a standard method for assessing platelet function.
  • Accurate platelet count adjustment is crucial for reliable LTA results.
  • Current methods for platelet count adjustment may affect aggregation responses.

Purpose of the Study:

  • To evaluate physiological saline (PS) and platelet-poor plasma (PPP) as diluents for adjusting platelet counts in LTA.
  • To determine the optimal method for platelet count adjustment across various agonists.

Main Methods:

  • Blood samples from 36 healthy individuals were used.
  • Platelet-rich plasma (PRP) was diluted with PS or PPP at ratios of 1.5, 2, 2.5, and 3.
  • Platelet aggregation was induced by adenosine diphosphate (ADP), arachidonic acid (ARA), collagen (COL), epinephrine (EPI), or ristocetin (RIS).
  • Maximal aggregation rates (MAs) were compared between dilution methods and agonists.

Main Results:

  • PS-adjusted PRP showed no significant change in MAs for ADP, ARA, COL, or EPI compared to original PRP.
  • PPP-adjusted PRP showed decreased MAs for ADP, ARA, and EPI.
  • PS-adjusted PRP showed decreased MA for RIS, while PPP-adjusted PRP did not.
  • No significant difference in RIS-induced MA was observed between whole blood-obtained-PPP and PRP-obtained-PPP.

Conclusions:

  • PS is recommended for adjusting platelet counts for LTA assays involving ADP, ARA, COL, and EPI.
  • Whole blood-obtained-PPP is a convenient alternative for RIS-induced aggregation.
  • These findings provide guidance for optimizing platelet function testing protocols.