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[Growth Regulation of Factor Inhibiting Hypoxia-Inducible Factor in Renal Carcinoma Cells]

Shi Deng1, Bo Yang1, Zheng-Ju Ren1

  • 1Department of Urology,Institute of Urology,West China Hospital,Sichuan University,Chengdu 610041,China.

Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition
|May 9, 2018
PubMed
Abstract

Insights

Factor Inhibiting Hypoxia-Inducible Factor (FIH-1) impacts renal carcinoma cell growth. Overexpressing FIH-1 reduced cell proliferation and key signaling pathway components in OS-RC-2 cells.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Renal carcinoma is a significant health concern.
  • Hypoxia-inducible factor (HIF) signaling is implicated in cancer progression.
  • Factor Inhibiting Hypoxia-Inducible Factor (FIH-1) is a key regulator of HIF.

Purpose of the Study:

  • To investigate the expression of FIH-1 in renal carcinoma cells.
  • To determine the effects of FIH-1 on cell growth and the HIF pathway.

Main Methods:

  • Real-time PCR and Western blot were used to assess gene and protein expression.
  • FIH-1 was overexpressed in OS-RC-2 and 786-0 renal carcinoma cell lines.
  • Cell proliferation was evaluated using MTT assays.

Main Results:

  • FIH-1, HIF-1α, HIF-2α, and VEGF were detected in OS-RC-2 cells; FIH-1, HIF-2α, and VEGF in 786-0 cells.
  • FIH-1 overexpression increased FIH-1 levels and decreased HIF-1α, HIF-2α, and VEGF in OS-RC-2 cells.
  • FIH-1 transfection inhibited OS-RC-2 cell proliferation but not 786-0 cell proliferation.

Conclusions:

  • The FIH-1/HIF-1α/VEGF signaling pathway is present in renal carcinoma cell lines.
  • FIH-1 plays a role in regulating the growth of certain renal carcinoma cells.
Keywords:
Factor inhibiting hypoxia-inducible factor-1Hypoxia inducible factorRNA interferenceRenal cell carcinoma

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