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Updated: Feb 10, 2026

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
Published on: July 30, 2016
Somatic Cell Reprogramming Informed by the Oocyte
Elena Gonzalez-Munoz1,2,3, Jose B Cibelli1,4,5
11 LARCEL, Andalusian Laboratory of Cell Reprogramming (LARCel), Andalusian Center for Nanomedicine and Biotechnology-BIONAND , Málaga, Spain .
Abstract:
The successful production of animals and embryonic stem cells using somatic cell nuclear transfer (SCNT) has demonstrated the unmatched nuclear reprogramming capacity of the oocyte and helped prove the degree of plasticity of differentiated cells. The introduction of transcription factors to generate induced pluripotent stem cells (iPSCs) displaced SCNT and, due to its ease of implementation, became the method of choice for cell reprogramming. Nonetheless, iPSC derivation remains inefficient and stochastic. This review article focuses on using the oocyte as a source of reprogramming factors, comparing the SCNT and iPSC mechanisms for remodeling chromatin and acquiring pluripotency.
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