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Changes in deciduous and permanent dentinal tubules diameter after several conditioning protocols: In vitro study
María de Los Angeles Moyaho-Bernal1, Rosalía Contreras-Bulnes1, Laura Emma Rodríguez-Vilchis1
1Universidad Autónoma del Estado de México, Facultad de Odontología, Centro de Investigación y Estudios Avanzados en Odontología (CIEAO), Jesús Carranza Esq. Paseo Tollocan, Col. Universidad, Toluca, Estado de México, C.P. 50130, México.
Microscopy Research and Technique
|May 9, 2018
Summary
Different dental conditioning protocols significantly impact dentin tubule diameter. Deciduous dentin shows wider tubule openings than permanent dentin across all tested methods, necessitating tailored approaches for pediatric and adolescent dental treatments.
Area of Science:
- Dental Materials Science
- Biomaterials Engineering
- Cariology and Operative Dentistry
Background:
- Current conditioning protocols for permanent dentin are established, but data for deciduous dentin is limited.
- Understanding dentinal tubule diameter is crucial for effective adhesion and treatment outcomes in restorative dentistry.
- Deciduous dentin's unique properties may influence the efficacy of various conditioning techniques.
Purpose of the Study:
- To compare the in vitro diameter of deciduous and permanent dentinal tubules after exposure to diverse conditioning protocols.
- To determine if specific conditioning methods yield different results on primary versus permanent dentin.
- To provide evidence-based recommendations for selecting appropriate conditioning protocols for pediatric and adolescent patients.
Main Methods:
- Eighty dentin samples (deciduous and permanent) were divided into sixteen groups (n=5 per group).
- Conditioning methods included acid etching, self-etch adhesive, and Er:YAG laser irradiation at varying energy densities (200 mJ and 300 mJ).
- Some groups received laser irradiation followed by phosphoric acid or self-etch adhesive.
- Scanning electron microscopy (SEM) was used to analyze dentin surfaces, and tubule diameters were measured using Image Tools software.
- One-way ANOVA was employed for statistical analysis (p ≤ 0.05).
Main Results:
- Deciduous dentin tubules ranged from 1.52 ± 0.32 µm to 3.88 ± 0.37 µm in diameter.
- Permanent dentin tubules ranged from 1.16 ± 0.16 µm to 2.76 ± 0.28 µm in diameter.
- All tested conditioning protocols resulted in significantly wider dentinal tubules in deciduous dentin compared to permanent dentin.
- Specific protocols showed more pronounced effects on primary dentin.
Conclusions:
- Deciduous and permanent dentin exhibit distinct responses to conditioning protocols, particularly regarding tubule diameter.
- Tailored conditioning strategies are necessary for deciduous dentin to optimize clinical success in pediatric dentistry.
- The findings highlight the importance of considering dentin age when selecting dental restorative materials and techniques.
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