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[Effect of vanadate on the vacuolar ATPase of Acer pseudoplatanus]
F Montrichard1, T Magnin, A Pugin
1Laboratoire de Biochimie, UA CNRS 531, Faculté des Sciences et des Techniques, Besançon.
Comptes Rendus Des Seances De La Societe De Biologie Et De Ses Filiales
|January 1, 1988
Abstract:
The ATPase of vacuoles isolated from Acer pseudoplatanus cells is strongly inhibited by vanadate, a specific inhibitor of plasma membrane ATPase. The degree of inhibition depends upon the ionic composition, ATP and magnesium concentrations of the reaction medium and the inhibition is reversed by EDTA which complexes vanadate. In absence of factor which may interfere with the effectiveness of inhibitor, vanadate inhibits the ATPase non-competitively.