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Semisynthesis, Characterization and Evaluation of New Adenosine Derivatives as Antiproliferative Agents
Francisco Valdés Zurita1, Nelson Brown Vega2,3, Margarita Gutiérrez Cabrera4
1Institute of Chemistry of Natural Resources, University of Talca, 3460000 Talca, Chile. franciscovaldesz@hotmail.es.
Abstract:
We describe the semisynthesis and biological effects of adenosine derivatives, which were anticipated to function as agonists for the A₃ receptor. Molecular docking was used to select candidate compounds. Fifteen nucleoside derivatives were obtained through nucleophilic substitutions of the N⁶-position of the nucleoside precursor 6-chloropurine riboside by amines of different origin. All compounds were purified by column chromatography and further characterized by spectroscopic and spectrometric techniques, showing moderate yield. These molecules were then evaluated for their antiproliferative activity in human gastric cancer cells expressing the A₃ receptor. We found that the compounds obtained have antiproliferative activity and that new structural modifications can enhance their biological activity. The ADME (Absorption, Distribution, Metabolism and Excretion) properties of the most active compounds were also evaluated theoretically.
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